primary antibodies include rabbit anti dnajc7 (Proteintech)
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primary antibodies include rabbit anti dnajc7
Primary Antibodies Include Rabbit Anti Dnajc7, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+include+rabbit+anti+dnajc7/DNAJC7+Antibody/pmc12993198-301-0-5
Average 93 stars, based on 14 article reviews
Primary Antibodies Include Rabbit Anti Dnajc7, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+include+rabbit+anti+dnajc7/DNAJC7+Antibody/pmc12993198-301-0-5
Average 93 stars, based on 14 article reviews
primary antibodies include rabbit anti dnajc7 - by Bioz Stars,
2026-10
93/100 stars
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1) Product Images from "The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation"
Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation
Journal: The Journal of Biological Chemistry
doi: 10.1016/j.jbc.2026.111292
Figure Legend Snippet: A CRISPRi screen of molecular chaperones identifies DNAJC7 as a modifier of polyQ aggregation. A , workflow of CRISPRi screen to identify molecular chaperone modifiers of polyQ aggregation in NLS-FRET-Q79 cells. Created with BioRender. B , volcano plot CRISPRi screen results, highlighting DNAJ protein and proteasomal subunit hits. C , flow cytometry plots of NLS-FRET-Q79 cells at 4 days of doxycycline with or without proteasomal inhibitor (carfilzomib). D and E , Western blot ( D ) and quantification ( E ) of DNAJC7 levels in NLS-FRET-Q79 cells expressing nontargeting control (NTC) sgRNAs or sgRNAs targeting DNAJC7. Each lane represents lysate from an independent well; bars and error bars indicate mean ± SD, and points represent n = 3 wells. Signal intensities were normalized to total protein and then to sgNTC#1. F , results of flow cytometry measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with sgNTC or sgDNAJC7. Data are presented as fold change relative to the mean of the sgNTCs for each independent experiment. Bars and error bars represent mean ± SD, and points represent n = 3 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). CRISPRi, CRISPR interference. NLS, nuclear localization signal; polyQ, polyglutamine; sgRNA, single guide RNA.
Techniques Used: Flow Cytometry, Western Blot, Expressing, Control, Transduction, CRISPR
Figure Legend Snippet: DNAJC7 suppresses mutant Huntingtin exon 1 (HTTex1) aggregation and colocalizes with inclusions. A , schematic of a lentiviral construct encoding a doxycycline-inducible eGFP-tagged fragment of HTTex1 containing 72 glutamines (GFP-HTTex1-Q72) used to generate a monoclonal HEK293T CRISPRi cell line. B , fluorescence micrograph of GFP-HTTex1-Q72 cells at 7 days of doxycycline treatment before and after treatment with Triton X-100. C , results of flow cytometry experiments measuring the fraction of total events containing detergent-resistant GFP + cells comparing those stably transduced with NTC or DNAJC7-targeting sgRNAs and normalized to the mean of NTCs. Data represent mean ± SD from n = 4 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). D , fluorescence micrographs of HEK293T cells 48 h after transient cotransfection of GFP-HTTex1-Q72 and either mTagBFP2 (BFP) alone or BFP-tagged DNAJC7. Arrows indicate cytoplasmic aggregates. E , flow cytometry plotting GFP-height versus GFP-width (pulse shape analysis) of HEK293T cells transfected with GFP-HTTex1-Q72 for 48 h. The detergent-resistant population of cells is designated as aggregate positive (Agg + ). F , results of flow cytometry experiments measuring Agg + cells in HEK293T cells 48 h after transient cotransfection with GFP-HTTex1-Q72 and either BFP or BFP-DNAJC7. Bars and error bars represent mean ± SD from n = 4 independent biological replicates (indicated by color), with each performed in technical triplicate. ∗ p < 0.05 by Student’s t test using mean values of technical replicates. The scale bars represent 10 μm. CRISPRi, CRISPR interference; eGFP, enhanced GFP; HEK293T, human embryonic kidney 293T cell line; NTC, nontargeting control; sgRNA, single guide RNA.
Techniques Used: Mutagenesis, Construct, Fluorescence, Flow Cytometry, Stable Transfection, Transduction, Cotransfection, Transfection, CRISPR, Control
Figure Legend Snippet: Chaperone CRISPRi screening reveals limited suppression of polyG aggregation despite effects by DNAJC7 overexpression. A , volcano plot showing results of a CRISPRi screen for molecular chaperone modifiers of polyG aggregation in the NLS-FRET-G100 cell line. B , pairwise comparison of gene scores between the polyG screen (from A ) and the polyQ screen ( B ). C , results of flow cytometry experiments measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with NTC or DNAJC7. Data represent mean ± SD from n = 3 independent experiments. D , representative fluorescence micrographs of HEK293T cells transiently cotransfected with NLS-mScarlet- and NLS-mNeonGreen-tagged uN2C-G100 together with either BFP or BFP-DNAJC7; only the mNeonGreen channel is shown. The scale bar represents 10 μm. E , flow cytometry quantification of the percentage of FRET-high cells relative to total cells expressing both mNeonGreen and mScarlet. Bars indicate mean ± SD from n = 3 independent experiments. ∗ p < 0.05 by Student’s t test. CRISPRi, CRISPR interference; HEK293T, human embryonic kidney 293T cell line; NLS, nuclear localization signal; NTC, nontargeting control; polyG, polyglucine; polyQ, polyglutamine; sgRNA, single guide RNA.
Techniques Used: Over Expression, Comparison, Flow Cytometry, Transduction, Fluorescence, Expressing, CRISPR, Control
Related Articles
Flow Cytometry:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Western Blot:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Expressing:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Control:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Transduction:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( CRISPR:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Mutagenesis:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Construct:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Fluorescence:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Stable Transfection:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Cotransfection:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Transfection:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Over Expression:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Comparison:Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation Article Snippet: Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.Membranes were washed 3 × 5 min with 1x Tris-buffered saline with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( Article Title: The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation. Article Snippet: Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.Membranes were washed 3x 5 min with 1x TBS with 0.1% Tween.. Primary antibodies include rabbit anti-DNAJC7 ( |